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Variation in human T cell receptor Vβ and Jβ repertoire: analysis using anchor polymerase chain reaction

✍ Scribed by William M. C. Rosenberg; Paul A. H. Moss; John I. Bell

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
959 KB
Volume
22
Category
Article
ISSN
0014-2980

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✍ Claudio Lunardi; Christopher Marguerie; Alex K. So 📂 Article 📅 1992 🏛 Springer-Verlag 🌐 English ⚖ 492 KB

We have studied the diversity of the expressed human T-cell receptor (TCR) beta-chain repertoire by analysis of mRNA from unstimulated peripheral blood T-cells. The anchored-polymerase chain reaction (PCR) was used to isolate TCRB transcripts. Of 20 full or near full-length functional transcripts se