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Variation in human acrocentric chromosomes with acridine orange reverse banding

✍ Scribed by Verma, Ram S. ;Lubs, Herbert A.

Publisher: Springer-Verlag
Year: 1975
Weight: 642 KB
Volume: 30
Category: Article
ISSN: 0018-7348
DOI: 10.1007/bf00279188

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✦ Synopsis

Twenty-five normal subjects were studied by acridine orange reverse (RFA) banding in order to obtain a preliminary estimate of the type and frequency of variations in color and length. Color variations were classified into 1 of 6 colors and size variations into 1 of 5 levels. The same cells were also studied by Q banding. Acridine orange reverse banding was found to be more useful than Q banding for characterizing variations in chromosomes 14, 15, 21 and 22. In addition, it was found that there was no consistent relationship between pale or bright Q banding and the various colors observed with RFA banding. For the optimal characterization of a chromosomal variation, multiple banding technics, including RFA banding, are necessary.

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Size variation polymorphisms of the short arm of human acrocentric chromosomes determined by R-banding by fluorescence using acridine orange (RFA)

✍ Ram S. Verma; Harvey Dosik; Herbert A. Lubs 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 180 KB

One hundred normal Caucasians were studied by the RFA technique to estimate the frequencies of size variation of the short arm of acrocentric chromosomes. Each size variation was classified into one of five levels. The most frequent size level(code) was 3; therefore, this was regarded as the 'averag