Variation in cellulase-constituting components from Trichoderma reesei with agitation intensity

✍ Scribed by Sukekuni Mukataka; Nobuyuki Kobayashi; Seigo Sato; Joji Takahashi

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 392 KB
• Volume: 32
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260320606

✦ Synopsis

Cellulase producing activity o f Trichoderma reesei

OM9414 was examined under various agitation intensities and at the dissolved oxygen concentration above 3 ppm. The producing activity greatly depended upon the agitation intensity, and the dependence on the agitation was different for each cullulase-constituting component. The maximum producing activities of FPA, CMCase, and P-glucosidase were obtained under different agitation conditions, 1.0, 0.7, and 1.4 m/s in tip velocity, respectively. Intensive agitation brought about remarkable reduction i n a l l cellulase components. The mycelial transformation through agitation intensity was also observed. Comparatively m i l d agitation of 0.3-1 .O m / s caused pellet formation as the culture progressed, although the pelletization was delayed with increasing agitation intensity. The behavior of the pelletization did not occur at 1.3 and 1.7 m/s throughout the course of cultivation, and under the latter agitation condition hyphae were broken up into short fragments. The cellulase producing activity is discussed in relation to such morphological changes.