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Variants within the 5′-flanking regions of bovine milk protein genes: I. κ-casein-encoding gene

✍ Scribed by T. A. Schild; V. Wagner; H. Geldermann

Publisher
Springer
Year
1994
Tongue
English
Weight
496 KB
Volume
89
Category
Article
ISSN
0040-5752

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✦ Synopsis

In order to identify DNA variants within the 5'-flanking region of the bovine κ-casein (κCn)-encoding gene, this area of the gene from 13 cows belonging to seven breeds (Holstein Friesian, Brown Swiss, German Simmental, Jersey, Galloway, Scottish Highland and Ceylon Dwarf Zebu) was analysed. For each individual, about 1 kb of the 5'-flanking region including exon I was amplified by polymerase chain reaction (PCR). The biotinylated PCR product was immobilized on magnetic beads followed by direct bidirectional sequencing using an automated DNA sequencer. Fifteen DNA variants were identified, some of which are located within potential regulatory sites and possibly involved in the expression of the κ-casein encoding gene.

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DNA variants within the 5′-flanking regi
DNA variants within the 5′-flanking region of milk-protein-encoding genes II. The β-lactoglobulin-encoding gene
✍ V. A. Wagner; T. A. Schild; H. Geldermann 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 835 KB

For the detection of polymorphisms within the 5'-flanking region of the β-lactoglobulin (-LG) -encoding gene a nucleotide sequence containing 795 bp of the promoter and 59 bp of exon I was cloned and sequenced. After comparing the sequence from the DNA of 11 diverse cows (different breeds and milk-p