Variable region correlates of group b allotypes: amino acid sequence studies of b9 L chains from homogeneous antibodies

✍ Scribed by B. A. Fraser; A. L. Thunberg; T. J. Kindt

• Book ID: 102826749
• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 562 KB
• Volume: 8
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830080603

✦ Synopsis

Abstract

The amino acid sequence was determined for residues 1 to 88 and residues 110 to 147 for a rabbit light chain (4153‐I) with allotype b9 from a homogeneous anti‐streptococcal antibody. The amino acid sequence of the L chain from a second antibody (4153‐II) was also determined for residues 1 to 49 and 62 to 77. In spite of the large differences in constant region sequence between b4 and b9 L chains, the variable regions of these antibodies are quite similar to those reported for b4 L chains. Both chains bear a b9‐specific substitution (glutamic acid) at position 16. The 4153‐I chain also has substitutions at positions 70 and 81 that may be exclusive to variable regions of the L chains with the b9 allotype. These allotype‐associated V~L~ structural differences offer support to the notion that structural genes for the C~L~ region are either linked to distinct V~L~ gene complexes or that certain V regions are expressed only in concert with certain C~L~ regions.