Valproic acid intoxication identified by 1H and 1H-13C correlated NMR spectroscopy of urine samples

✍ Scribed by Azaroual, N

• Publisher: Springer
• Year: 2000
• Tongue: English
• Weight: 151 KB
• Volume: 10
• Category: Article
• ISSN: 0968-5243
• DOI: 10.1016/s1352-8661(00)00086-7

✦ Synopsis

Analysis of biological fluids by proton and carbon nuclear magnetic resonance spectroscopy ( 1 H and 13 C NMR) is a promising tool in clinical biology. We used this method for rapid toxicological screening in the case of two suicide attempts. For each case, a urine sample was analysed at 300 MHz by 1D and 2D sequences (TOCSY and HMBC) in a short experimental time. Quantification was performed by peak integration on the 1D 1 H NMR spectrum. For the two patients, results showed the same resonances of the major metabolite, valproyl-O-glucuronide at concentrations of 121 and 44 mmol/l.