Abstract
Valinomycin is shown to form a stable complex with protons in the form of H~3~O^+^ ions. Using ^1^H and ^13^C nuclear magnetic resonance (NMR), Fourier transform (FT) infrared spectroscopy, and ab initio–density functional theory (DFT) quantum mechanical calculations, it is shown that H~3~O^+^, produced by hydrogen bis(1,2‐dicarbollyl) cobaltate (HDCC) in the presence of water, interacts with valinomycin in 1,1,2,2‐tetrachloroethane‐d~2~ to give a relatively stable complex. The equilibrium constant K of the complex formation was derived from chemical shifts in ^1^H‐NMR, its value being 5.9. The proton affinity constant estimated from this value taking into account HDCC dissociation is 10^5.3^, i.e., high enough to be relevant under physiological conditions. Under suitable conditions there is a fast exchange of H~3~O^+^ between valinomycin molecules, the exchange correlation time being of the order of 10^−4^ s. The instantaneous structure of the complex is slightly asymmetric, the H~3~O^+^ ion being strongly hydrogen‐bonded to three of the six ester carbonyl groups and weekly bound to the residual ones by electrostatic interactions. This asymmetry is averaged by fast reorientation and displacement of H~3~O^+^ so that the molecule appears to be symmetric in the time window of NMR. The results indicate that valinomycin could serve as a carrier for proton transfer across a biological membrane. © 2006 Wiley Periodicals, Inc. Biopolymers 82: 536–548, 2006
This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]