Validity of thyroglobulin mRNA assay in peripheral blood of postoperative thyroid carcinoma patients in predicting tumor recurrences varies according to the histologic type : Results of a prospective study
✍ Scribed by Rocco Bellantone; Celestino Pio Lombardi; Maurizio Bossola; Angela Ferrante; Pietro Princi; Mauro Boscherini; Ludovica Maussier; Massimo Salvatori; Vittoria Rufini; Francesca Reale; Luisa Romano; Giovanni Tallini; Giovanni Zelano; Alfredo Pontecorvi
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 93 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
The objective of the current study was to evaluate the ability of serum thyroglobulin mRNA assay in detecting local and distant recurrences in patients who underwent surgery for thyroid carcinoma.
METHODS.
Sixty-six consecutive patients were studied. One year after surgery, all patients underwent clinical examination and radioiodine scan, and a blood sample was taken for serum thyroglobulin (Tg) immunoassay and for Tg mRNA assay by reverse transcription-polymerase chain reaction (RT-PCR). RNA was extracted from cells pellet and analyzed by RT-PCR using specific primers for Tg.