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Validation of RNA interference in western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults

✍ Scribed by Murugesan Rangasamy; Blair D Siegfried


Book ID
112179777
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
126 KB
Volume
68
Category
Article
ISSN
1526-498X

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Acetylcholinesterase (AChE, EC 3.1.1.7) was purified from western corn rootworm (WCR, Diabrotica virgifera virgifera) beetles by affinity chromatography. The purification factor reached over 20,000-fold with a specific activity of 169.5 mumol/min/mg and a yield of 23%. The Vmax values for hydrolyzin