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Validation of reference genes for real-time quantitative PCR studies in gene expression levels ofLactobacillus caseiZhang

✍ Scribed by Wenjing Zhao; Yan Li; Pengfei Gao; Zhihong Sun; Tiansong Sun; Heping Zhang

Publisher: Springer-Verlag
Year: 2010
Tongue: English
Weight: 304 KB
Volume: 38
Category: Article
ISSN: 1476-5535
DOI: 10.1007/s10295-010-0906-3

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## Abstract Careful validation of reference genes used for the normalization of real‐time RT‐PCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t