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Validation of reference genes for qPCR studies on Caco-2 cell differentiation

✍ Scribed by Claudia Piana; Michael Wirth; Stefan Gerbes; Helmut Viernstein; Franz Gabor; Stefan Toegel


Book ID
116437954
Publisher
Elsevier Science
Year
2008
Tongue
French
Weight
130 KB
Volume
69
Category
Article
ISSN
0939-6411

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Real-time RT-PCR method was exploited to identify endogenous reference genes in differentiating human T helper cells. When using this technology in our experimental system, finding a set of genes whose mRNA expression levels would not change appeared to be very challenging. Our initial plan to use t