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Validation of a reliable set of primer pairs for measuring gene expression by real-time quantitative RT-PCR in equine leukocytes

✍ Scribed by M.D. Figueiredo; C.E. Salter; A.L.P. Andrietti; M.L. Vandenplas; D.J. Hurley; J.N. Moore


Book ID
116966532
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
326 KB
Volume
131
Category
Article
ISSN
0165-2427

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## Background Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles is accurate normalisation. In most of the studies published on real-time PC