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Validation of a differential PCR and an ELISA procedure in studying HER-2/neu status in breast cancer

✍ Scribed by Pilar F. Valerón; Ricardo Chirino; Leandro Fernandez; Santiago Torres; Domingo Navarro; José Aguiar; Juan J. Cabrera; Bonifacio N. Diaz-Chico; Juan C. Diaz-Chico

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
674 KB
Volume
65
Category
Article
ISSN
0020-7136

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✦ Synopsis

HER-2lneu oncogene status and total cellular p I 8SHER-' content were simultaneously analyzed in 4 I 5 invasive breast-cancer specimens by differential PCR and ELISA respectively. Mathematical analysis of the data led us to establish a cut-off value of I .7 for the ratio between the intensity of the HER-2lneu gene band and the reference gene band, to consider the HER-2lneu gene amplified, and of 260fmollmg protein, to consider p I 8SHER-' over-expressed. Of the 415 tumors studied, 15% showed a diverse degree of HER-2lneu gene amplification. Of these tumors, 87% showed over-expression of the p18SHER-'. Of the remaining 352 specimens that did not display HER-2lneu gene amplification, 97% showed no p I 8SHER-' over-expression ( p < 0.OOOi). In 40 selected samples with a p18SHER-' level

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