Validation and long-term assessment of an approach for the high throughput determination of lipophilicity (log POW) values using multiplexed, absorbance-based capillary electrophoresis
✍ Scribed by Kit-Sum Wong; Jeremy Kenseth; Roy Strasburg
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 246 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
A critical evaluation of the use of 96-capillary multiplexed microemulsion electrokinetic chromatography (MMEEKC) for the indirect determination of octanolwater partition coefficients (log P OW values) for a wide range of structurally different compounds is presented. The various components of the microemulsion solution were evaluated and optimized for use in a multiplexed capillary format. A six-component calibration mixture and 23 different solutes (n ¼ 4 each) were analyzed simultaneously, providing a throughput of up to 46 samples/h, which translates to greater than a 20-fold improvement over existing indirect log P OW methods. Agreement to within AE0.5 log P units of literature values was obtained for 51 of the 54 tested neutral and basic (uncharged) solutes. A linear free energy relationship (LFER) analysis performed on the MMEEKC system supports its use as a viable and effective model of the classical shakeflask method for log P OW determinations. Moreover, a standard deviation of 0.1 or less log P units was obtained for 35 of 36 solutes analyzed repeatedly over an 8-month time period, documenting the long-term effectiveness of the analysis format. Critical comparisons between the proposed MMEEKC method and existing separation methods for the indirect determination of log P OW values are also made. Overall, the results indicate that 96-capillary MMEEKC can serve as a high throughput, cost effective and robust approach and as a valid model for log P OW determinations.