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Validating internal controls for quantitative plant gene expression studies

✍ Scribed by Amy M Brunner; Igor A Yakovlev; Steven H Strauss


Book ID
115007891
Publisher
BioMed Central
Year
2004
Tongue
English
Weight
277 KB
Volume
4
Category
Article
ISSN
1471-2229

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## Abstract The stability of expression of an internal control is required for accurate and reliable normalization in quantitative real‐time reverse transcription–polymerase chain reaction (qRT‐PCR) experiments. However, expression of commonly used reference genes can be regulated under specific ex