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Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets

✍ Scribed by Marta de Diego; Marcia Avello; Sigrid Mennickent; Marcos Fernández; Pola Fernández

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
425 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

In the present study, an RP high performance liquid chromatographic method was developed and validated for the determination of allicin in garlic powder and tablets. Chromatographic separation was carried out on an RP‐18~e~ column (125 mm×4 mm), using a mobile phase, consisting of methanol–water (50:50 v/v), at a flow rate of 0.5 mL/min and UV detection at 220 nm. Ethylparaben was used as the internal standard. The assay was linear for allicin concentrations of 5.0–60.0 μg/mL. The RSD for precision was <6.14%. The accuracy was above 89.11%. The detection and quantification limits were 0.27 and 0.81 μg/mL, respectively. This method was used to quantify allicin in garlic powder samples. The results showed that the method described here is useful for the determination of allicin in garlic powder and tablets.

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