Vacuum-blotting: a new simple and efficient transfer of proteins from sodium dodecyl sulfate—polyacrylamide gels to nitrocellulose
✍ Scribed by M. Peferoen; R. Huybrechts; A. De Loof
- Book ID
- 118323560
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 360 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0014-5793
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📜 SIMILAR VOLUMES
A method is described for analyzing calcium/calmodulin-dependent protein kinase activity in crude or purified samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to PVDF membrane. The blotted protein is denatured in situ with guanidine \
Standard mixtures of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Polynomial regression analysis was used to fit curves to the data points obtained by plotting log,, of protein molecular weight versus electrophoretic mobility. Polynomials with orders ranging