Vaccinia Virus: Methods and Protocols

✍ Scribed by Jason Mercer

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 318
• Series: Methods in Molecular Biology 2023
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book provides practical information for the laboratory that can be applied to the study of vaccinia and other poxviruses while emphasizing long-standing field standards and focusing on emerging new technologies applied in the field of poxvirology. The methods and protocols have been designed with the bench scientist in mind, being presented in a fashion that makes them useful for both starting and veteran poxvirus researchers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Vaccinia Virus: Methods and Protocols serves as a valuable resource for scientists looking to bring new methods and procedures into their lab in order to make exciting discoveries that will continue to deepen our understanding of this fascinating virus family.

✦ Table of Contents

Front Matter ....Pages i-xv
Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections with Poxviruses (Stuart N. Isaacs)....Pages 1-27
Bioinformatics for Analysis of Poxvirus Genomes (Shin-Lin Tu, Chris Upton)....Pages 29-62
Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis (Rachel L. Roper)....Pages 63-71
Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins (N. Bishara Marzook, Timothy P. Newsome)....Pages 73-92
Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines (Amber B. Rico, Annabel T. Olson, Matthew S. Wiebe)....Pages 93-108
Vaccinia Virus Genome Editing Using CRISPR (Carmela Di Gioia, Ming Yuan, Yaohe Wang)....Pages 109-117
RNAi-Mediated Depletion of Poxvirus Proteins (Caroline Martin, Samuel Kilcher)....Pages 119-130
Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation (Nouhou Ibrahim, Paula Traktman)....Pages 131-141
Preliminary Screening and In Vitro Confirmation of Orthopoxvirus Antivirals (Douglas W. Grosenbach, Dennis E. Hruby)....Pages 143-155
Vaccinia Virus Transcriptome Analysis by RNA Sequencing (Shuai Cao, Yongquan Lin, Zhilong Yang)....Pages 157-170
Ribosome Profiling of Vaccinia Virus-Infected Cells (Yongquan Lin, Wentao Qiao, Zhilong Yang)....Pages 171-188
Quantitative PCR-Based Assessment of Vaccinia Virus RNA and DNA in Infected Cells (Moona Huttunen, Jason Mercer)....Pages 189-208
Click Chemistry-Based Labeling of Poxvirus Genomes (Harriet Mok, Artur Yakimovich)....Pages 209-220
Visualizing Poxvirus Replication and Recombination Using Live-Cell Imaging (Quinten Kieser, Patrick Paszkowski, James Lin, David Evans, Ryan Noyce)....Pages 221-235
High-Content Analyses of Vaccinia Plaque Formation (Artur Yakimovich, Jason Mercer)....Pages 237-253
Super-resolution Microscopy of Vaccinia Virus Particles (Robert Gray, David Albrecht)....Pages 255-268
Bioluminescence Imaging as a Tool for Poxvirus Biology (Beatriz Perdiguero, Carmen Elena Gómez, Mariano Esteban)....Pages 269-285
Growth and Purification of Vaccinia Virus Stocks for MPM Imaging (Glennys V. Reynoso, John P. Shannon, Jeffrey L. Americo, James Gibbs, Heather D. Hickman)....Pages 287-299
Intravital Imaging of Vaccinia Virus-Infected Mice (John P. Shannon, Olena Kamenyeva, Glennys V. Reynoso, Heather D. Hickman)....Pages 301-311
Back Matter ....Pages 313-315

✦ Subjects

Biomedicine; Virology