v-erbB oncogene expression accounts for most variations in protein synthesis after avian erythroblastosis virus infection of chicken embryo fibroblasts: A two-dimensional electrophoresis study
✍ Scribed by Christelle Desbois; Olivier Gandrillon; Jacques Samarut; Dr. Jean-Jacques Madjar
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 796 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
v-erbB Oncogene expression accounts for most variations in protein synthesis after avian erythroblastosis virus infection of chicken embryo fibroblasts: A two-dimensional electrophoresis study
The effect of the v-erbA and/or v-erbB oncogenes on cellular gene expression was investigated after separation by two-dimensional polyacrylamide gel electrophoresis of [3'S]methionine-labelled proteins from chicken embryo fibroblasts (CEF), infected by either the avian erythroblastosis virus (AEV) carrying both oncogenes, or by viruses carrying only one of them. We observed significant changes in the synthesis of 34 proteins in AEV-transformed CEF as compared with control cells.The synthesis of 24 of them was increased while the synthesis of the other 10 proteins was decreased.The expression of v-erbB alone is necessary and sufficient to induce changes in the synthesis of 27 proteins while the 7 remaining modifications are observed only in cells expressing v-erbB togetherwith v-erbA. Moreover, the deregulation of protein synthesis by v-erbB-expressing viruses was correlated with the morphological transformation state of cells.