UV resonance Raman excitation profiles of l-cystine

✍ Scribed by Craig R. Johnson; Sanford A. Asher

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 464 KB
• Volume: 18
• Category: Article
• ISSN: 0377-0486
• DOI: 10.1002/jrs.1250180509

✦ Synopsis

The UV Raman excitation profiles of Ccystine have been measured between 220 and 514.5 nm. No enhancement is observed from the lowest energy electronic transition (A,,, =245 nm). The excitation profiles for the SS, C-S and C=O stretching vibrations are each satisfactorily modeled by standard Albrecht A-term pre-resonance expressions which indicate that the Raman intensities derive from a state@) at ca 170 nm which is probably u -P u* in character. Selective enhancement of disulfide stretching vibrations in proteins will require excitation in the vacuum UV spectral region.