UV Resonance Raman Determination of Protein Acid Denaturation: Selective Unfolding of Helical Segments of Horse Myoglobin †

Structural study on acid-induced unfoldi

Structural study on acid-induced unfolding intermediates of myoglobin by using UV resonance Raman scattering from tryptophan residues

✍ Shinji Hashimoto; Jyun-ichi Fukasaka; Hideo Takeuchi 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB

## Abstract Ultraviolet resonance Raman spectra of sperm whale myoglobin were examined at various pH in the presence and absence of a helix‐stabilizing anion, SO~4~^2−^. Sperm whale myoglobin contains two Trp residues (Trp7 and Trp14) in helix A. The 229 nm excited Raman spectra of metmyoglobin and