UV irradiation of chromosomal DNA and its effect upon MPF and meiosis in mammalian oocytes
✍ Scribed by Dr. J. Bradshaw; T. Jung; J. Fulka Jr.; R. M. Moor
- Book ID
- 102952734
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 990 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1040-452X
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✦ Synopsis
Abstract
Bovine oocytes were irradiated at germinal vesicle (GV) or metaphase II (MI) stage, after Hoechst staining, with chromosomally focused UV‐C (254 nm) or UV‐A (> 330 nm). UV‐C irradiation at GV stage did not inhibit germinal vesicle breakdown (GVBD) or chromosomal condensation; spindle formation was abolished and maturation promoting factor (MPF) levels failed to increase. UV‐A irradiation at GV stage caused meiotic arrest at anaphase I; MPF levels were lower than control. UV‐C irradiation at MII stage led to subsequent abnormal parthenogenetic activation when MPF levels failed to decrease. A normal male but no female pronucleus was formed at fertilization. UV‐A irradiation at MII stage also caused abnormal activation; MPF levels declined normally. A normal male and abnormal female pronucleus formed at fertilization. UV‐A irradiation results have implications for oocyte evaluation during development using Hoechst staining. UV‐C irradiation is a potential means for oocyte enucleation in nuclear transfer. © 1995 Wiley‐Liss, Inc.