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Utilizing Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy to Probe the Structure of Membrane Proteins

✍ Scribed by Mayo, Daniel J.; Zhou, Andy; Dev Sahu, Indra; McCarrick, Robert M.; Walton, Parker J.; Ring, Adam C.; Troxel, Kaylee R.; Coey, Aaron T.; Hawn, Jaclyn M.; Emwas, Abdul-Hamid; Lorigan, Gary A.


Book ID
119208739
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
36 KB
Volume
100
Category
Article
ISSN
0006-3495

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✍ Daniel Mayo; Andy Zhou; Indra Sahu; Robert McCarrick; Parker Walton; Adam Ring; πŸ“‚ Article πŸ“… 2011 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 178 KB

## Abstract A new approach has been developed to probe the structural properties of membrane peptides and proteins using the pulsed electron paramagnetic resonance technique of electron spin echo envelope modulation (ESEEM) spectroscopy and the α‐helical M2Ξ΄ subunit of the acetylcholine receptor in

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An cleclron spin echo envelope modulahon lrequency analysis is performed ol the 14N and 15N bacleriochlorophyll a\* canon in sohd solution Hyperfine couphngs of 2 0 and 3.1 MHz arc derived which correspond to the perpendicular component ol Lhe axial hyperfine coupling tensor. Seven quadrupole lreque