Utilization of zeolite Y in the removal of anionic, cationic and nonionic detergents during purification of proteins

✍ Scribed by Zoltan Blum; H»kan Eriksson

• Book ID: 104644824
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 382 KB
• Volume: 5
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152755

✦ Synopsis

Hydrophobic zeolite Y was used to adsorb detergents from protein solutions and within one minute the commonly used detergents sodium dodecyl sulfate, cetyl trimethyl ammonium bromide, and Triton X-100 at concentrations of 10 mg/ml were adsorbed to a level below their critical micelle concentrations. From the detergent depleted solutions 77 to 85 % of the proteins were recovered; the lower value was obtained with protein concentration below one mg/ml.