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Utilization of volatile fatty acids in ruminants. IV. Relative activities of acetyl CoA synthetase and acetyl CoA hydrolase in mitochondria and intracellular localization of acetyl CoA synthetase

โœ Scribed by Quraishi, Shahida; Cook, Robert M.


Book ID
126171501
Publisher
American Chemical Society
Year
1972
Tongue
English
Weight
434 KB
Volume
20
Category
Article
ISSN
0021-8561

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On the Assay of Acetyl-CoA Synthetase Ac
โœ P.G. Roughan; J.B. Ohlrogge ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 488 KB

Acetyl-CoA synthetase activity in vitro is assayed quickly and conveniently by incubating whole chloroplasts, chloroplast extracts, or leaf extracts with labeled acetate, CoA, ATP, and Mg and transferring aliquots of the reaction mixture to pieces of either Whatman No. 1 or DE81 filter paper. Unreac