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Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from a petroleum-contaminated soil

Publisher
Elsevier Science
Year
2002
Weight
159 KB
Volume
43
Category
Article
ISSN
0140-6701

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✦ Synopsis

The Center for Coal Utilization, Japan (CCUJ) has promoted clean coal technology programs to develop innovative coal conversion technology to clean energy, form the viewpoint of countermeasures for global warming issues and maintenance of energy security in Japan. The di-Me ether (DME) synthesis technology has been developed as oil alternative technology and development of hydrogen manufacturing technology with nearly super critical water state is underway. DME is expected to become a substitute for propane gas in wide application fields or for diesel fuel. A 5 ton/day bench-scale plant successfully synthesized DME by a charging coal bed with methane and/or a model coal gasification gas. The advanced hydrogen production process utilizes water at high pressure and high temperature to decompose coal in order to produce hydrogen at high concentration Commercialization of these technologies is necessary for coping with future environmental issues and realizing clean power systems using coal.

02/01742

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## Abstract The extracellular endopeptidase synthesized by soil bacterium __Pseudomonas fluorescens__ was purified to homogeneity in a four‐step procedure. The enzyme was purified 45‐fold, with a 20% recovery. The endopeptidase appeared to be a monomer with a molecular mass of approx. 50 kDa. The e