Utilization of methoxylated benzoates and formation of intermediates byDesulfotomaculum thermobenzoicumin the presence or absence of sulfate

✍ Scribed by Masaharu Tasaki; Yoichi Kamagata; Kazunori Nakamura; Eiichi Mikami

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 361 KB
• Volume: 157
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00245151

✦ Synopsis

Desulfotomaculum thermobenzoicum strain TSB (DSM 6193) was found to utilize some methoxylated benzoates as carbon and energy source with or without sulfate. 3- or 4-Methoxybenzoate, vanillate (4-hydroxy-3-methoxybenzoate), syringate (3,5-dimethoxy-4-hydroxybenzoate) and 3,4,5-trimethoxybenzoate were converted to corresponding hydroxybenzoates. However, neither 2-methoxybenzoate nor 2,6-dimethoxybenzoate was utilized. The organism grew acetogenically on each of the methoxylated benzoates in the absence of sulfate. 3,4-Dihydroxy-5-methoxybenzoate was detected during conversion of syringate, and syringate and 3,4-dihydroxy-5-methoxybenzoate were detected during conversion of 3,4,5-trimethoxybenzoate as intermediates. These findings indicate that 4-methoxyl-group is most readily cleaved, whereas 2-methoxyl-group is not utilized by the organism.