Utilization of cellulose from waste paper by Myrothecium verrucaria

✍ Scribed by David M. Updegraff

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 792 KB
• Volume: 13
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260130106

✦ Synopsis

Extensive screening studies on cellulolytic bacteria and fungi led to the selection of Myrothecium v e m m r i a as the organism producing the maximum rate of protein biosynthesis from ball-milled newspaper. Studies in aerated stirred-jar fermentom were carried out to determine the conditions for maximum protein synthesis rate and maximum final protein concentration. The optimum aeration rate was 250 to 374 mM of oxygen at 300 to 400 rpm stirring rate. The pH optimum was broad, from 3.9 to 6.5. Urea at 0.03% and yeast autolysate a t 0.1% stimulated growth rate and protein production. The, maximum rate of protein biosynthesis and the maximum protein yield were 0.3 glliterjday and 1.42 g/liter, respectively, from medium G3 with 4% ball-milled newspaper. The final product, obtained by evaporation of the total culture, was 33.7 g from one liter of medium which originally contained 40 g of ball-milled newspaper and 11.3 g of other dissolved materials. The protein content of this final product was 3.3 g, calculated from total organic N X 6.25 or 1.42 g calculated from the biuret method. Both the synthesis rate and the final cell yield are below those obtainable by growing Fungi Imperjecti, yeasts or bacteria on soluble materials such as glucose.

5,418422 (1968).