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Utilization of a PCR-based strategy for the isolation of cDNA clones encoding the human α3(IX) chain

✍ Scribed by Brewton, R.; Wood, B.; Ren, Z-X.; Lee, B.; Horton, W.; Baker, J.; Mayne, R.

Book ID
122058749
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
101 KB
Volume
14
Category
Article
ISSN
0945-053X

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Cytochrome P-450 monooxygenases are membrane-bound enzymes involved in a wide range of biosynthetic pathways in plants. An efficient PCR strategy for isolating cytochrome P-450 cDNA clones from plant cDNA libraries is described. A set of degenerate primers for PCR amplification was designed to recog