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Utilization of a DNA enzyme immunoassay for the detection of proviral DNA of human immunodeficiency virus type 1 by polymerase chain reaction

✍ Scribed by Zella, Davide; Cavicchini, Ada; Cattaneo, Ercole; Cimarelli, Andrea; Bertazzoni, Umberto


Book ID
123232672
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
559 KB
Volume
3
Category
Article
ISSN
0928-0197

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## Abstract A highly sensitive two‐step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV‐1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna