Utilisation des gels d'acrylamide-agarose souos forme de perles et de plaques pour le fractionnement des protéines sériques
✍ Scribed by M Delay; E Boschetti; R Tixier; M Dugué; F Rousselet
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 773 KB
- Volume
- 92
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Use of acrylamide-agarose gel beads and plates in the study of serum. proteins
Gel filtration on acrylamide-agarose beads AcA 34 allows the fractionation of serum proteins according to their molecular weights. The proteins contained in each fraction are analysed by electrophoresis on acrylamide-agarose plates and identified by immunoelectrophoresis.
The elution diagram shows three main peaks with a plateau between the first peak and the second peak and a shoulder at the beginning of the second peak. The authors have been able to characterize p-lipoprotein and IgM in the first peak, a,-macroglobulin at the level of the plateau, siderophilin, ceruloplasmin and haemopexin between the second peak and the third peak, and albumin and oc,-antitrypsin in the third peak. Orosomucoid and prealbumin are eluted last. The IgA range extends from the first peak to the second peak and the IgG are localized between the shoulder of the second peak and the third peak. The haptoglobin polymers are distributed all along the elution diagram according to their molecular weights. Gel filtration on acrylamide-agarose beads combined with electrophoresis on acrylamide-agarose plates and immunoelectrophoresis should provide a particularly convenient and efficient analytical tool for the study of pathological sera.