𝔖 Bobbio Scriptorium
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Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

✍ Scribed by Remy, Ingrid; Ghaddar, Galia; Michnick, Stephen W


Book ID
109946305
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
358 KB
Volume
2
Category
Article
ISSN
1750-2799

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Protein fragment complementation assays (PCAs) based on different reporter proteins have been described as powerful tools for monitoring dynamic protein-protein interactions in living cells. The present study describes the construction of a PCA system based on genetic splitting of TEM-1 beta-lactama