Using live FRET imaging to reveal early protein–protein interactions during T cell activation
✍ Scribed by Tomasz Zal; Nicholas RJ Gascoigne
- Book ID
- 104014347
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 285 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0952-7915
No coin nor oath required. For personal study only.
✦ Synopsis
The emerging challenge for proteomics in general and lymphocyte biology in particular is to understand protein-protein interactions in the dynamic context of the living cell. Particularly interesting are the molecular dynamics of the T cell receptor-CD3 complex and other immunoreceptors in immune synapses. Fluorescence (or Fo ¨rster) resonance energy transfer (FRET) is one of the few techniques that are capable of giving dynamic information about the nanometer-range proximity between molecules, as opposed to simply the subcellular co-localization that is provided by fluorescence microscopy. Spectral changes in fluorescence intensity and down modulation of donor lifetime are the basis for rapidly developing approaches to real-time FRET imaging. With two-photon excitation, FRET can now be extended to in vivo imaging.