𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Using disaccharides to enhance in vitro and in vivo transgene expression mediated by a lipid-based gene delivery system

✍ Scribed by Wen-Chi Tseng; Chien-Hsiang Tang; Tsuei-Yun Fang; Ling-Yu Su

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
191 KB
Volume
9
Category
Article
ISSN
1099-498X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background Lipid‐based vectors have been widely applied to in vivo and in vitro gene delivery. Disaccharides can effectively stabilize lipid membranes. This study examined whether disaccharides could enhance the transgene expression mediated by lipid‐based vectors.

Methods Different disaccharides were incorporated into the vectors prepared with DOTAP/protamine/DNA (LPD) or with DNA/cationic liposomes containing DOTAP, DOTAP/Chol, DOTAP/DOPE, or DC‐Chol/DOPE. The levels of transgene expression and internalized plasmid of CHO cells were represented by the percentages of GFP‐positive cells and the fluorescence intensity of ethidium‐monoazide covalently labeled plasmid, respectively. The vectors containing either cellobiose or trehalose were also intravenously injected into mouse tail vein to investigate the potentials of in vivo applications.

Results For enhancing the transgene expression, cellobiose was found to be effective for all the vectors whereas maltose decreased the effectiveness of DOTAP/Chol liposomes and LPD. For the internalization of plasmid, most disaccharides were able to increase the cellular delivery of DOTAP, DOTAP/Chol, and DOTAP/DOPE liposomes, but caused decreases in the cellular entry of DC‐Chol/DOPE liposomes. An approximately linear correlation between the internalized plasmid and the transgene expression was observed for all the treatments in this study. When the vectors were administered to mouse by intravenous injection, 10‐fold and 3‐fold increases in the luciferase expression of lung were observed for DOTAP liposomes containing 330 mM cellobiose and trehalose, respectively.

Conclusions This study showed that using trehalose and cellobiose with a lipid‐based delivery system provides a straightforward approach to effectively enhance both in vitro and in vivo transgene expression. Copyright © 2007 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Enhanced in vitro and in vivo cationic l
Enhanced in vitro and in vivo cationic lipid-mediated gene delivery with a fluorinated glycerophosphoethanolamine helper lipid
✍ Otmane Boussif; Jérôme Gaucheron; Caroline Boulanger; Catherine Santaella; Hanno 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB

## Background: One of the main drawbacks of synthetic, non-viral gene vectors is their relatively low in vivo efficiency when compared with viral vectors. the present paper describes the use of a partially fluorinated glycerophosphoethanolamine (f-pe), a close analog of dope, which, as a helper lip

Sustained transgene expression in cartil
Sustained transgene expression in cartilage defects in vivo after transplantation of articular chondrocytes modified by lipid-mediated gene transfer in a gel suspension delivery system
✍ Henning Madry; Magali Cucchiarini; Ute Stein; Klaus Remberger; Michael D. Menger 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 277 KB

## Abstract ## Background Genetically modified chondrocytes may be able to modulate articular cartilage repair. To date, transplantation of modified chondrocytes into cartilage defects has been restricted to viral vectors. We tested the hypothesis that a recombinant gene can be delivered to sites