Stable Expression of a Synthetic Gene fo
โ
Christopher W. Carreras; Michael A. Siani; Daniel V. Santi; Susan B. Dillon
๐
Article
๐
2002
๐
Elsevier Science
๐
English
โ 88 KB
A synthetic gene for the human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in HEK293 cells with the bioluminescent Ca 2ุ indicator protein aequorin. The dosedependent response of the receptor to motilin was demonstrated using transient transfections, a