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Useful modification of Pauly's reagent : Detection of low-molecular-weight histidine-containing peptides by isoelectric focusing

✍ Scribed by M. Faupel; E. Von Arx


Book ID
104144035
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
560 KB
Volume
157
Category
Article
ISSN
1873-3778

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✦ Synopsis


An improved method for the detection of low-molecular-weight (< 10,000 daltons) hi&line-containing peptides on isoelectric focusing_is described.

The major advantage of the method is that the staining mixture (a mod&&on of Pauly's reagent) does not react with ampholytes of similar size and PI, permitting accurate determination of the isoelectric points of these pep.tides.

KNTRODUCX'ION