Useful clinical assays for vitamin D metabolites
โ Scribed by Clemens, Thomas L.
- Book ID
- 122724062
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 686 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1043-2760
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๐ SIMILAR VOLUMES
The fluorogenic dienophile 1,2,4-triazoline-3,5-dione with a highly fluorescent quinoxalinone group at the 4-position (DMEQ-TAD) was synthesized and exploited as a reagent to assay vitamin D metabolites. 25-Hydroxyvitamin D3, 1 alpha,25-dihydroxyvitamin D3, and 24(R),25-dihydroxyvitamin D3 reacted q
We describe a multiple assay of the three main vitamin D metabolites, \(250 \mathrm{HD}, 24,25(\mathrm{OH})_{2} \mathrm{D}\), and \(1,25(\mathrm{OH})_{2} \mathrm{D}\), in \(0.5 \mathrm{ml}\) of serum, which does not require high-performance liquid chromatography. The assay involves extracting the se