Use ofURA3as a reporter of gene expression inC. albicans
β Scribed by Kristi K. Myers; Paul S. Sypherd; William A. Fonzi
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 951 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Vectors containing fusions of the Candida albicans ACT promoter to heterologous genes were constructed and transformed into a C. albicans host strain. beta-Galactosidase (Lac4p) activity was detected in transformants carrying an ACT fusion to the Kluyveromyces lactis LAC4 gene, while fusions to the
Bacterial luciferase can be assayed rapidly and with high sensitivity both in vivo and in vitro. Here we demonstrate that the N-terminal hydrophobic domain of the alpha catalytic subunit of the luciferase enzyme is indispensable for enzyme activity, although N-terminal translational fusions with ful