Use of toluene/Triton X-100 scintillation mixture for counting C14-protein radioactivity
โ Scribed by N.P. Madsen
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 176 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
Sucrose and cesium chloride gradient centrifugation are extremely widely used techniques for the fractionation of RNA and ribonucleoprotein particles. A variety of methods have been used to separate high molecular-weight material from gradient fractions as a preliminary to scintillation counting (l-
The counting rate of [ 1-r4C] trichloroacetic acid (TCA) was not stable in a standard toluene/Triton X-100 liquid scintillation solution because this compound becomes partially degraded to 14C0, and CHCl,. Both toluene and Triton were contributing factors in causing this degradation. The NCS solubil