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Use of the Tn903neomycin-resistance gene for promoter analysis in the fission yeastSchizosaccharomyces pombe

✍ Scribed by Christine Lang-Hinrichs; Claudia Dössereck; Isabelle Fath; Ulf Stahl

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
803 KB
Volume
18
Category
Article
ISSN
0172-8083

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✦ Synopsis

The bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro amino-glycoside phosphotransferase activity. On the other hand, the heterologous promoter of the Saccharomyces cerevisiae alcohol dehydrogenase I gene (adc1) is shown to be a weak promoter in Schizosaccharomyces pombe, though its activity is significantly enhanced in cells grown on glycerol as a carbon source. This system for selection and detection of promoter-active sequences may provide a useful basis for the analysis of promoter elements in fission yeast.

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