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Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

✍ Scribed by Arne Skerra


Book ID
116132519
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
573 KB
Volume
151
Category
Article
ISSN
0378-1119

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Fed-batch fermentation for production of a single-chain Fv antibody fragment (scFv) expressed as a recombinant periplastic protein from Escherichia coli was investigated. A high cell density of 50 g dry cell weight per liter was routinely achieved in a 14-L vessel by controlled exponential feeding o