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Use of the RFLP-PCR diagnostic test for characterizing MACE and kdr insecticide resistance in the peach potato aphid Myzus persicae

✍ Scribed by Stefano Cassanelli; Barbara Cerchiari; Sara Giannini; Davide Bizzaro; Emanuele Mazzoni; Gian Carlo Manicardi

Book ID
105359591
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
132 KB
Volume
61
Category
Article
ISSN
1526-498X

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✦ Synopsis

Abstract

The peach‐potato aphid Myzus persicae (Sulzer) has developed a number of insecticide resistance mechanisms owing to the high selective pressure produced by world‐wide insecticide treatments. Knowledge of the geographical distribution and the temporal evolution of these resistant phenotypes helps to develop suitable pest‐management programs. Current understanding of the major mechanisms of resistance at the molecular level makes it possible to diagnose the presence of modified acetylcholinesterase (MACE) or knockdown resistance (kdr). This paper describes a rapid method for the identification of both resistance mechanisms in a single molecular assay by using restriction fragment length polymorphism of PCR products (RFLP‐PCR) in individual as well as pooled aphids. Copyright © 2004 Society of Chemical Industry

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The peach-potato aphid Myzus persicae (Sulzer) can resist a range of insecticides by over-producing detoxifying esterase and having mutantinsensitive forms of the target proteins, acetylcholinesterase (AChE), and the sodium channel. Using a combination of bioassays, biochemical and DNA diagnostics,