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Use of the perfused rat heart to study cardiac metabolism: retrospective and prospective views

โœ Scribed by J. R. Williamson; K. Kobayashi


Publisher
Springer
Year
1984
Tongue
English
Weight
635 KB
Volume
79
Category
Article
ISSN
0300-8428

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A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P