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Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans

✍ Scribed by Isabella Buttino; Adrianna Ianora; Ylenia Carotenuto; Valerio Zupo; Antonio Miralto

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 513 KB
Volume: 60
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.10284

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Confocal Laser Scanning Microscope techniques have been applied to study the developmental biology of marine copepods and decapod larvae. The lipophylic probes DiI and DiOC~6~ were used to study both the external and internal morphology of these crustaceans, whereas the same DiOC~6~ and the specific nuclear probe Hoechst 33342 were used to study embryonic development of copepods in vivo. To distinguish viable from non‐viable copepod embryos, the vital dye dichlorodihydrofluorescein diacetate (H~2~DCFDA) was used. Major advantages and difficulties in the use of these non‐invasive techniques in studies of the reproductive biology of marine crustaceans are discussed. Microsc. Res. Tech. 60:458–464, 2003. © 2003 Wiley‐Liss, Inc.

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