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Use of the complex ratio method in wide-range measurement devices

โœ Scribed by O. A. Tcybulskii

Book ID
120749907
Publisher
Springer US
Year
2013
Tongue
English
Weight
104 KB
Volume
56
Category
Article
ISSN
0543-1972

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A radioenzymatic method suitable for the estimation of nanomole amounts of a wide range of metabolites is described. The method involves the conversion of [ l-'4C]a-ketoglutarate to [ l-'4C]glutamate by NADH or NADPH generated stoichiometrically in preliminary reactions specific for the metabolite u