Use of the Airfuge for analysis and preparation of receptors incorporated into liposomes: Studies with the receptor for immunoglobulin E

✍ Scribed by Benjamin Rivnay; Henry Metzger

• Book ID: 102984852
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 635 KB
• Volume: 130
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90626-7

✦ Synopsis

A Beckman Airfuge has been employed for studying the interaction between lipids and the receptor for immunoglobulin E (IgE). For analytic experiments, samples were applied underneath a discontinuous sucrose gradient. After a 30-min centrifugation in a fixed-angle rotor, liposomes floated toward the top of the gradient whereas unincorporated receptor-IgE complexes remained at the bottom of the tube. Liposomes with incorporated receptors were also efficiently separated in the ACR-90 preparative rotor. These methods of "Airfuge flotation" can provide useful adjuncts to more traditional methods for density-gradient centrifugation especially when rapid analysis of small samples is desired.