Use of tandem mass spectrometry (MS-MS) for aldosterone assay at the nanogram level in complex biological mixtures

✍ Scribed by Danielle Prome; Antoinette Viger; Andrée Marquet

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 491 KB
• Volume: 172
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90441-1

✦ Synopsis

Aldosterone was derivatized

as butane boronate cyclic ester. This derivative gives an intense molecular ion under 70-eV electron impact conditions. This ion exhibits an abundant metastable decomposition in the second field free region by loss of formic acid, which can be used for the assay. A linear relationship was observed between the ionic current and the amount of aldosterone from IO to 200 ng. A good correlation between this new method and the radioimmunological assay is observed. Aldosterone can be readily detected at the nanogram level.