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Use of synthetic peptides and copolymers to study the substrate specificity and inhibition of the protein tyrosine kinase pp60c-src

✍ Scribed by Raymond J.A. Budde; Nihal U. Obeyesekere; Shi Ke; John S. McMurray


Book ID
113269308
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
621 KB
Volume
1248
Category
Article
ISSN
0167-4838

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Solid-phase ELISAs for the determination of EGF receptor (EGF-R) and pp60c-src tyrosine protein kinase activity are described. The methods were developed and optimized using purified recombinant EGF-R intracellular domain (ICD) and pp60c-src tyrosine protein kinases. A standardized assay that utiliz