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Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins

✍ Scribed by Santosh Noronha; Jeanne Kaufman; Joseph Shiloach

Book ID
110257366
Publisher
Springer
Year
1999
Tongue
English
Weight
201 KB
Volume
8
Category
Article
ISSN
1573-8272

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## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i