The rates of cell attachment of the anchorage-dependent mammalian cell line Vero t o the gelatin-based macroporous microcarrier Cultispher-G were determined under various conditions. An optimal rate of attachment (0.98 x min-') occurred by an intermittent stirring regimen of 3 min stirring at 40 rpm
Use of recombinant and synthetic peptides as attachment factors for cells on microcarriers
β Scribed by James Varani; Dennis R. Inman; Suzanne E. G. Fligiel; William J. Hillegas
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 1020 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0920-9069
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## Abstract A working system for studying the effects of factors involved in the chemical nature of microcarriers on cell attachment, spreading, and growth was established. The system is based on polyacrylamide beads, prepared by the emulsion polymerization technique. Sieved beads of desirable mean